These findings are highly relevant to the enhanced regenerative activities of P-15-coated implants. However, attachment as well as stable adhesion can also be mediated by specific, high affinity receptor-ligand interactions, e.g., between cell surface receptors like integrins and their ECM binding partner(s) on the substratum. Cell attachment is the earliest phase of cell adhesion and can be mediated by physical forces like charge-charge, Van der Waals bonding or hydrophobic interactions between cell surfaces and the substratum. Therefore, it was concluded that P-15-coated surfaces promote cell attachment. Within minutes of adding cells to P-15-coated substrata, those cells had attached in greater numbers , were more resistant to being rinsed away and exhibited increased viability and a lower level of apoptosis compared to cells that had been added to control substrata. P-15 promotes cell attachment. For many cell types, attachment to various pro-adhesive substrata typically occurs within minutes to several hours following their addition to a substratum e.g. A brief summary of the key biological activities is summarised in the subsequent sections. Numerous studies over the last twenty years investigated P-15’s biological activities on cells in vitro. Cells have a natural high affinity for P-15 which increases the number of viable cells at the healing site. i-FACTOR’s mechanism of action is surface bound. The novel “Attract, Attach, Activate” mechanism of action of action leads to enhanced, predictable, and proven bone formation (Figure 2)įigure 2. The release of growth factors further accelerates the bone regeneration process by promoting the migration, proliferation, and differentiation of mesenchymal stem cells and other bone progenitor cells from surrounding tissue. ![]() P-15 also improves cell viability and the receptor-mediated anchorage of osteoblasts by P-15 initiates a number of signal transduction pathways that lead to the synthesis of growth factors, cytokines, and bone morphogenetic proteins. The P-15 peptide in i-FACTOR is immobilized on an anorganic bone mineral (ABM) surface and enhances cell adhesion and migration, and induces osteoblast cell proliferation and differentiation. The ability of P-15 to enhance cell binding, increases the number of viable cells at the healing site and speeds the process of new bone formation, and closely resembles the natural process of bone regeneration. I-FACTOR Bone Graft increases the opportunity for cell binding in the fusion site by making an abundance of the P-15 peptide available to osteogenic cells. The P-15 sequence (residues 769-783) comprises about one per cent of the length of the collagen molecule. Type I collagen molecules comprise a triple helix formed from two α(I) and one α2(I) chains. Schematic illustrating the organic phase of bone, comprised of rope-like collagen macromolecules, their component triple helices, and P-15, a 15 amino acid peptide present within single chains of the triple helices. These cells bind to the synthetic P-15 found in i-FACTOR the same way they would bind to human Type I collagen (figure 1).įigure 1. This protein segment (P-15) is responsible for the attachment and proliferation of osteogenic cells. 2016 14:103.I-FACTOR Peptide Enhanced Bone Graft is based upon the biological activity of a 15 amino-acid peptide found in type I collagen. The role of peptides in bone healing and regeneration: a systematic review. Pountos I, Panteli M, Lampropoulos A, Jones E, Calori GM, and Giannoudis PV. Visser R, Rico-Llanos GA, Pulkkinen H, and Becerra J. I-FACTOR Bone Graft is an advanced biologic that is supported with level I evidence, satisfies the highest regulatory imperatives, and provides a better cost/benefit ratio than engineered growth factors and cellular (stem cell) allografts.ġ. i-FACTOR provides a mechanism of action that is safe and predictable. Unlike engineered growth factors, i-FACTOR Bone Graft is not a morphogen and, being surface-bound, all cellular activity resulting from P-15 attachment is restricted to the implant surface so bone cannot grow where it doesn’t belong (ectopic bone growth). The unique combination creates a surface bound mechanism of action, which provides a microenvironment capable of supporting cell adhesion, proliferation and differentiation that enhances the body’s natural bone healing process. ![]() I-FACTOR Peptide Enhanced Bone Graft is the only osteobiologic that combines a small bioactive peptide, P-15, which replicates the cell-binding domain of human type I collagen, with an anorganic bone mineral (ABM).
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |